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KMID : 1007520220310070843
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Food Science and Biotechnology
2022 Volume.31 No. 7 p.843 ~ p.847
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Prediction and identification of new type holin protein of Escherichia coli phage ECP26
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Park Do-Won
Lee Jong-Hun
Park Jong-Hyun
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Abstract
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Bacteriophages (phages) infecting specifically target bacteria utilize a unique lysis module known as the holin-endolysin cassette to release progeny. Studies on the phage lytic proteins could contribute to the development of alternatives to antibiotics. Here, we predicted and identified the holin protein of rV5-like phage ECP26 for increasing lytic activity of the phage endolysin. In silico analysis revealed that open reading frame 151 (ORF151) of ECP26 contained two transmembrane domains. Co-expression of endolysin with ORF151 resulted in the cell lysis of Escherichia coli, suggesting that ORF151 protein functioned as the holin that disrupted the cytoplasmic membrane. The putative holin showed a high amino acid homology by more than 80% to the predicted holins of rV5-like phages. Therefore, the holin protein would be helpful for developing efficient lysis strategies with endolysin against gram-negative E. coli.
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KEYWORD
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Bacteriophage, Holin, rV5-like phage, Escherichia coli
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